Simultaneous determination of six isoflavonoids in commercial Radix Astragali by HPLC-UV

A HPLC-UV method for the quantification of six major isoflavonoids, calycosin 7-O-β-d-glucoside (1), formononetin 7-O-β-d-glucoside (2), (6αR, 11αR) 3-hydroxy-9,10-dimethoxypterocarpan-3-O-β-d-glucoside (3), 7,2′-dihydroxy-3′,4′-dimethoxyisoflavan-7-O-β-d-glucoside (4), calycosin (5) and formononetin (6), in Radix Astragali (Huangqi) was developed and validated. The method was proven to be sensitive, specific, accurate and precise, as well as effective and easy.