کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9019 615 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Conformational recovery and preservation of protein nature from heat-induced denaturation by water-soluble phospholipid polymer conjugation
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Conformational recovery and preservation of protein nature from heat-induced denaturation by water-soluble phospholipid polymer conjugation
چکیده انگلیسی

The effect of water-soluble phospholipid polymer conjugated to a protein on conformational change during heat-quenched stress was investigated in this study. Well-defined pyridine disulfide end-functional poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) was synthesized by well controlled atom transfer radical polymerization method. Synthesized PMPC was the site specifically conjugated to a protein, and the effect on conformational change during heat-quenched stress was estimated by circular dichroism and fluorescence study. As a result, a single phospholipid polymer chain conjugated to a protein give a great effect on conformational preservation in both secondary and tertiary structures even after the heat-quenced process. Moreover, even the conformational recovery which gave the completely reversible conformational preservation was observed in circular dichroism study. The resulting protein activity was also confirmed, and no significant decline was induced by heat-quenched stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 28, October 2009, Pages 4859–4867
نویسندگان
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