کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9110841 | 1568751 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction of cytokines by heat shock proteins and concanavalin A in murine splenocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
There has been considerable interest in the effect of heat shock proteins (HSPs) on the innate immune system. Whether HSPs have any direct effects on the activation of lymphocytes is not clear. Using gene expression array, protein array and enzyme-linked immunosorbent assay, we demonstrated that highly purified recombinant murine Hsp60 (rmHsp60), essentially free of lipopolysaccharide contamination, had no effect in the expression of 113 cytokine genes and the release of 22 common cytokines including interferon-γ (IFN-γ) and interleukin-2 (IL-2) by murine splenocytes. Likewise, recombinant human Hsp60 (rhHsp60) and rhHsp70 had no effect in the release of IFN-γ and IL-2. In contrast, concanavalin A induced the expression of a number of cytokine genes and the release of IFN-γ and IL-2. These results suggest that Hsp60 and Hsp70 do not induce cytokine production by murine splenocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 32, Issues 3â4, November 2005, Pages 149-154
Journal: Cytokine - Volume 32, Issues 3â4, November 2005, Pages 149-154
نویسندگان
Yun Wang, Baochong Gao, Min-Fu Tsan,