کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9110979 | 1155382 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Comparison of biological activity of recombinant woodchuck interferon gamma and tumor necrosis factor alpha produced in baculovirus and Escherichia coli expression systems
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Comparison of biological activity of recombinant woodchuck interferon gamma and tumor necrosis factor alpha produced in baculovirus and Escherichia coli expression systems Comparison of biological activity of recombinant woodchuck interferon gamma and tumor necrosis factor alpha produced in baculovirus and Escherichia coli expression systems](/preview/png/9110979.png)
چکیده انگلیسی
The full-length cDNAs of recombinant woodchuck interferon gamma (rwIFNγ) and woodchuck tumor necrosis factor alpha (rwTNFα) were cloned into baculovirus transfer vectors and expressed in insect Sf9 cells. The recombinant proteins secreted by the insect cells, bac-rwIFNγ and bac-rwTNFα, were found to be functionally competent. Their biological activities were compared to those of rwIFNγ and rwTNFα produced in the Escherichia coli (E. coli) expression system. The bac-rwIFNγ demonstrated a 4.5-fold greater protective activity against encephalomyocarditis virus-induced cytolysis of woodchuck hepatocytes and that of class I MHC antigen presentation on the hepatocytes than rwIFNγ derived from E. coli. The bac-rwTNFα was cytotoxic towards murine fibroblasts and able to upregulate class I MHC antigen display and these effects were about 18-fold greater than those triggered by rwTNFα from E. coli at a comparable protein level. In addition, the antiviral activity of bac-rwIFNγ was inhibited by anti-wIFNγ antibodies and the cytotoxicity of bac-rwTNFα neutralized by cross-reactive antibodies to murine TNFα. The study showed that the expression of rwIFNγ and rwTNFα in the baculovirus system generated biologically active cytokines whose potency was considerably greater than those produced in E. coli.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytokine - Volume 30, Issue 1, 7 April 2005, Pages 22-34
Journal: Cytokine - Volume 30, Issue 1, 7 April 2005, Pages 22-34
نویسندگان
J. Wang, T.I. Michalak,