کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9113066 | 1156203 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Promoter activity of sea lamprey proopiocortin and proopiomelanotropin genes in AtT-20/D16v cells
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adrenocorticotropic hormone (ACTH) and melanophore-stimulating hormone (MSH) are produced in the pars distalis and pars intermedia, respectively, throughout vertebrates. These hormones together with β-endorphin are encoded on a single gene proopiomelanocortin (POMC) in gnathostomes, but in the sea lamprey, an agnathan, ACTH and MSH are encoded on two separate genes, proopiocortin (POC) and proopiomelanotropin (POM), respectively. Moreover, the nucleotide sequences of 5â²-flanking regions of the POC and POM genes are significantly different from each other. To investigate the potential promoter activities of the POC and POM genes, we constructed promoter reporter plasmids by fusing the 5â² flanking sequences (nucleotides â1151 to +31 and â2510 to +51, respectively) to a firefly luciferase gene. Transient transfection studies in AtT-20/D16v cells, which derived from a mouse pituitary tumor cell line, revealed that the 5â²-flanking sequence of the POC gene did not exhibit promoter activity, whereas that of the POM gene showed the activity at high levels nearly equivalent to SV40 promoter. Analysis of a series of the 5â²-deleted reporter for the POM gene in the AtT-20/D16v cells demonstrated that the 422 bp 5â²-flanking sequence was sufficient for promoter activity, while the sequence from â853 to â574 may contain negatively acting regulatory elements. Because the POC and POM genes are supposed to have differentiated from a common ancestor, during evolution, the POC gene may lack essential element(s) for expression in the AtT-20/D16v cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 144, Issue 2, November 2005, Pages 182-187
Journal: General and Comparative Endocrinology - Volume 144, Issue 2, November 2005, Pages 182-187
نویسندگان
Akiyoshi Takahashi, Osamu Nakata, Takayoshi Kosugi, Koichiro Kako, Takafumi Sakai, Michihiko Ito, Nobuhiko Takamatsu, Tadayoshi Shiba, Stacia A. Sower, Hiroshi Kawauchi,