کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9113220 | 1156217 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of hormonal status on enkephalin-degrading aminopeptidase activity in the HPA axis of female mice
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
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چکیده انگلیسی
Opioids are involved in the regulation of hypothalamus-pituitary-adrenal (HPA) axis activity under physiological conditions. In the present work, we analyzed the influence of ovariectomy and estradiol (E), progesterone (P) or estradiol plus progesterone (EÂ +Â P) replacement on soluble (S) and membrane-bound (MB) enkephalin-degrading aminopeptidase activity (EDA) in the HPA axis. Female mice (Balb/C) were distributed in 15 groups of 10 animals each: sham-operated controls (C), ovariectomized controls (OV-C), and ovariectomized mice treated with increasing doses of E (10, 20 or 40Â mg/kg), P (100, 200 or 400Â mg/kg) or EÂ +Â P (10Â +Â 100, 20Â +Â 200 or 40Â +Â 400Â mg/kg). In hypothalamus, ovariectomy increased both S and MB EDA activities, whereas E replacement returned them to control levels, although MB EDA activity increased again after the replacement with 40Â mg/kg E. P replacement increased S EDA activity, but returned MB EDA activity to control levels. The replacement of EÂ +Â P returned both S and MB EDA activities to control levels, although MB EDA activity was lower than control values after the replacement with 10Â +Â 100Â mg/kg EÂ +Â P. In pituitary, neither ovariectomy nor the replacement of E or EÂ +Â P changed S EDA, although the highest concentrations of P increased S EDA activity. However, ovariectomy increased MB EDA and E replacement returned the activity to control or below control levels, depending on the concentration used. However, P administration returned the activity to control or below control levels depending on the concentration used, although 200Â mg/kg P had no effects on MB EDA. EÂ +Â P replacement returned pituitary MB EDA activity to control levels. In adrenal glands, ovariectomy did change either S or MB EDA. However, E, P or EÂ +Â P replacement decreased S EDA activity in different degrees, depending of the dose administrated. No changes were detected in MB EDA after hormone replacement. These results indicate that female steroid hormones influence EDA activity at different levels of HPA axis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 141, Issue 2, April 2005, Pages 135-140
Journal: General and Comparative Endocrinology - Volume 141, Issue 2, April 2005, Pages 135-140
نویسندگان
M.J. GarcÃa-López, J.M. MartÃnez-Martos, M.D. Mayas, M.P. Carrera, M.J. RamÃrez-Expósito,