کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9121314 1159184 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus
چکیده انگلیسی
Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 252, Issue 1, 1 November 2005, Pages 51-56
نویسندگان
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