کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121396 | 1159186 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional regulation of the S-layer protein type I secretion system in Caulobacter crescentus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
The Gram-negative Caulobacter crescentus exports RsaA, the crystalline S-layer subunit protein using a dedicated type I secretion system. The protein and two transporter genes (rsaADE) are located together, comparable to the Escherichia coli type I hemolysin hlyCABD operon, where read through of a stem loop following hlyCA results in reduced transcription of the hlyBD. Using two genetic approaches and a direct assessment of transcription from regions 5â² to the genes we learned that rsaD and rsaE were transcribed together as a separate transcript from rsaA. These results are contrary to previous assumptions about the rsaADE type I secretion gene control and add another theme to the area of type I secretion transcription regulation. It may be that to accommodate the high levels of RsaA secretion, the type I transporters must be transcribed independently from rsaA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 251, Issue 1, 1 October 2005, Pages 29-36
Journal: FEMS Microbiology Letters - Volume 251, Issue 1, 1 October 2005, Pages 29-36
نویسندگان
Michael C. Toporowski, John F. Nomellini, Peter Awram, Assaf Levi, John Smit,