کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9121410 1159186 2005 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of novel Trichoderma hamatum genes expressed during mycoparasitism using subtractive hybridisation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Identification of novel Trichoderma hamatum genes expressed during mycoparasitism using subtractive hybridisation
چکیده انگلیسی
Subtractive hybridisation was used to target novel genes involved in the mycoparasitic interaction of the biocontrol agent Trichoderma hamatum with the phytopathogen Sclerotinia sclerotiorum. Nineteen novel T. hamatum genes were identified that showed increased expression during mycoparasitism compared to a T. hamatum control. Sequence analysis revealed some cDNA fragments had similarity to known fungal or bacterial genes whereas others had no similarity to any genes previously described. Only one of the novel genes has been characterised in another Trichoderma species, the Trichoderma reesei hex1gene. The proteins encoded by the novel genes included three monooxygenases, a metalloendopeptidase, a gluconate dehydrogenase, an endonuclease and a proton ATPase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 251, Issue 1, 1 October 2005, Pages 105-112
نویسندگان
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