کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121612 | 1159193 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum](/preview/png/9121612.png)
چکیده انگلیسی
An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) - a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and α-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 247, Issue 2, 15 June 2005, Pages 113-121
Journal: FEMS Microbiology Letters - Volume 247, Issue 2, 15 June 2005, Pages 113-121
نویسندگان
C. James Newbold, Neil R. McEwan, Roger E. Calza, Emilie N. Chareyron, Stéphane M. Duval, Sylvain C.P. Eschenlauer, Freda M. McIntosh, Nancy Nelson, Anthony J. Travis, R. John Wallace,