کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121743 | 1159196 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel β-glucanase gene from Bacillus halodurans C-125
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
To examine the activity of the gene product, the gene was cloned as a His-tagged protein and expressed in Escherichia coli. The purified enzyme showed activity against lichenan, barley β-glucan, laminarin, and carboxymethyl curdlan. Thin-layer chromatography showed that the enzyme hydrolyzes substrates in an endo-type manner. When β-glucan was used as a substrate, the pH optimum was between 6 and 8, and the temperature optimum was 60 °C. After 2 h incubation at 50 and 60 °C, the residual activity remained 100% and 50%, respectively. The enzymatic activity was abolished after 30 min incubation at 70 °C. Based on the results, the gene encodes an endo-type β-1,3(4)-d-glucanase (E.C. 3.2.1.6).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 248, Issue 1, 1 July 2005, Pages 9-15
Journal: FEMS Microbiology Letters - Volume 248, Issue 1, 1 July 2005, Pages 9-15
نویسندگان
Masatake Akita, Kinya Kayatama, Yuji Hatada, Susumu Ito, Koki Horikoshi,