کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121783 | 1159197 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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![عکس صفحه اول مقاله: Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production Overexpression of a hydrogenase gene in Clostridium paraputrificum to enhance hydrogen gas production](/preview/png/9121783.png)
چکیده انگلیسی
A [Fe]-hydrogenase gene (hydA) was cloned from Clostridium paraputrificum M-21 in Escherichia coli using a conserved DNA sequence of clostridial hydrogenase genes amplified by PCR as the probe. The hydA gene consisted of an open reading frame of 1749 bp encoding 582 amino acids with an estimated molecular mass of 64,560 Da. It was ligated into a shuttle vector, pJIR751, originally constructed for Clostridium perfringens and E. coli, and expressed in C. paraputrificum. Hydrogen gas productivity of the recombinant increased up to 1.7-fold compared with the wild-type. In the recombinant, overexpression of hydA abolished lactic acid production and increased acetic acid production by over-oxidation of NADH, which is required for reduction of pyruvic acid to lactic acid in the wild-type.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 246, Issue 2, 15 May 2005, Pages 229-234
Journal: FEMS Microbiology Letters - Volume 246, Issue 2, 15 May 2005, Pages 229-234
نویسندگان
Kenji Morimoto, Tetsuya Kimura, Kazuo Sakka, Kunio Ohmiya,