کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9121916 | 1159201 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas Evaluation of 16S rDNA- and gyrB-DGGE for typing members of the genus Aeromonas](/preview/png/9121916.png)
چکیده انگلیسی
The DNA sequence of the gyrB gene is a suitable phylogenetic marker for bacterial systematics. In this study, the diversity of Aeromonas spp. present in environmental samples was assessed by a PCR combined with DGGE approach. PCR primers targeting the gyrB gene of aeromonads were designed and the resulting amplicons were analyzed by DGGE. The gyrB-DGGE analysis was evaluated with Aeromonas isolates and reference strains allowing discrimination of the majority of strains. The gyrB-DGGE analysis is a powerful tool to monitor the presence and evaluate the diversity of aeromonads in complex samples, as is the case of water from a wastewater treatment plant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 246, Issue 1, 1 May 2005, Pages 11-18
Journal: FEMS Microbiology Letters - Volume 246, Issue 1, 1 May 2005, Pages 11-18
نویسندگان
Marta Tacão, Alexandra Moura, Artur Alves, Isabel Henriques, Maria José Saavedra, António Correia,