کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9122185 1159206 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Copper alone, but not oxidative stress, induces copper-metallothionein gene in Neurospora crassa
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Copper alone, but not oxidative stress, induces copper-metallothionein gene in Neurospora crassa
چکیده انگلیسی
Two metal response elements, flanking an antioxidant response element, were identified in regions upstream (−3730 bp) to copper metallothionein (CuMT) gene of Neurospora crassa. Presence of copper in culture media, but not of pro-oxidants like H2O2 or menadione, induced CuMT gene expression that could not be completely abolished by antioxidants such as N-acetyl cysteine and ascorbic acid. Gel shift assays revealed the ability of nuclear extracts from copper induced cultures to bind PCR-amplified metal response or antioxidant response elements. Similar observations could not be made with cultures exposed either to pro-oxidants or antioxidants. These results differentiate between CuMT gene induction by copper from antioxidant functions associated with the identified upstream elements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEMS Microbiology Letters - Volume 242, Issue 1, 1 January 2005, Pages 45-50
نویسندگان
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