کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127052 | 1160197 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and characterization of HIV-1-inducible astrocyte elevated gene-1, AEG-1
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کلمات کلیدی
ORFGSPAEG-1Ha-ras oncogeneESTs - EST هاgene specific primer - آغازگر مخصوص ژنtumor necrosis factor-α - تومور نکروز عامل αExpressed sequence tags - تگ های توالی بیان شدهHAD - داشته استHIV-associated dementia - دمانس مرتبط با HIVGlutamate excitotoxicity - سمیت تحریک گلوتاماتTNF-α - فاکتور نکروز توموری آلفاopen reading frame - قاب خواندن بازHIV-1 - ویروس اچ آی وی نوع یکhuman immunodeficiency virus-1 - ویروس نقص ایمنی بدن انسان 1astrocyte elevated gene-1 - ژن 1 افزایش یافته است
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We presently describe the full-length cloning and functional characterization of an HIV-1-inducible gene, astrocyte elevated gene (AEG)-1. Additionally, a novel method is outlined for producing tag-free recombinant protein in a baculovirus system and its use in producing AEG-1 protein. AEG-1 mRNA is expressed ubiquitously with higher expression in tissues containing muscular actin and its expression is increased in astrocytes infected with HIV-1 or treated with gp120 or tumor necrosis factor (TNF)-α. The mRNA encodes a single pass transmembrane protein of predicted molecular mass of 64-kDa and pI 9.3 that predominantly localizes in the endoplasmic reticulum and perinuclear region. Ectopic expression of AEG-1 inhibits excitatory amino acid transporter 2 (EAAT2) promoter activity with the potential to promote glutamate excitotoxicity and consequently HIV-1-associated dementia (HAD). AEG-1 expression is elevated in subsets of breast carcinomas, malignant gliomas and melanomas and it synergizes with oncogenic Ha-ras to enhance soft agar colony forming ability of non-tumorigenic immortalized melanocytes, documenting its tumor promoting activity. AEG-1 may affect tumor progression in multiple cell lineages by augmenting expression of the transformed phenotype and/or by inducing glutamate excitotoxicity in malignant glioma. In these contexts, an HIV-1-inducible gene, AEG-1, may contribute to multiple brain abnormalities, including HAD and tumor formation, by both common and distinct mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 353, Issue 1, 20 June 2005, Pages 8-15
Journal: Gene - Volume 353, Issue 1, 20 June 2005, Pages 8-15
نویسندگان
Dong-chul Kang, Zao-zhong Su, Devanand Sarkar, Luni Emdad, David J. Volsky, Paul B. Fisher,