کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127115 | 1569972 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The cellulose synthase gene PrCESA10 is involved in cellulose biosynthesis in developing tracheids of the gymnosperm Pinus radiata
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کلمات کلیدی
CSRUTRRSWCESAAngiospermsUDPRT-PCRcDNA - cDNADNA complementary to RNA - DNA مکمل RNAuridine diphosphate - uridine دی فسفاتEST - استExpressed Sequence Tag - تکرار اظهار نظرBase pair(s) - جفت پایه (ها)digoxigenin - دیگوکسین ژنDIG - شماEvolution - فرگشت Phylogenetic - فیلوژنتیکuntranslated region(s) - ناحیه (های) غیر مترادفReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ای
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
One full length (PrCESA10) and seven other cDNA clones (PrCESA2, 3, 5-8, 11) encoding cellulose synthases (CESAs) were isolated from the coniferous gymnosperm Pinus radiata. PrCESA10 encodes a protein predicted to contain the same domains and regions as angiosperm CESA genes: a zinc finger domain, a hypervariable region 1 (HVR1), a plant-conserved region (CR-P), a class-specific region or hypervariable region 2 (HVR2), in addition to the four conserved domains U1-U4 that are characteristic of the family 2 processive β-glycosyltransferases. The P. radiata protein is also predicted to contain eight transmembrane domains. The zinc finger domain, the CR-P and the C-terminal portion of the proteins, are highly conserved between P. radiata and the nearest angiosperm CESA protein from Solanum tuberosum. Reverse transcriptase-PCR showed that all the P. radiata genes were expressed in all organs tested, although to different extents. In situ hybridization studies with PrCESA10 in stems of 2- and 12-month-old seedlings showed that it was expressed in the secondary xylem in the two-to-three most recently developed tracheids, which were laying down secondary cell walls.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 350, Issue 2, 9 May 2005, Pages 107-116
Journal: Gene - Volume 350, Issue 2, 9 May 2005, Pages 107-116
نویسندگان
Erwin Krauskopf, Philip J. Harris, Jo Putterill,