کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127135 | 1160201 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and functional characterization of two apple S-adenosylmethionine decarboxylase genes and their different expression in fruit development, cell growth and stress responses
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کلمات کلیدی
ORFIBADAFSAMDCEtdBr2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسیدN6-benzylaminopurine - N6-بنزیلامینوپورینS-adenosylmethionine decarboxylase - S-adenosylmethionine dearboxylaseethidium bromide - اتیدیوم بروماید3-indolebutyric acid - اسید 3-اندولوباتیکSalt stress - تنش شوریFruit development - توسعه میوهdays after flowering - روز بعد از گلDIG - شماopen reading frame - قاب خواندن باز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Two full-length S-adenosylmethionine decarboxylase (SAMDC) cDNAs, MdSAMDC1 and MdSAMDC2, were isolated from apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.]. Both cDNAs encoded tiny and small ORFs in addition to the SAMDC ORFs, and genomic sequences of MdSAMDC1 and MdSAMDC2 contained two or three introns in the 5â² upstream regions, respectively. Yeast complementation experiment indicated that two MdSAMDCs encoded functional proteins, and that the tiny and small ORFs possibly repressed their translation efficiency. RNA gel blot analysis showed that MdSAMDC1 were differentially regulated in fruits depending on the developmental stage and in cell suspension during the culture period, but MdSAMDC2 did not. In contrast, MdSAMDC2 was positively induced by cold and salt stresses, but MdSAMDC1 was not. These results suggest that MdSAMDC1 is mainly involved in fruit development and cell growth while MdSAMDC2 in stress responses, compared with their respective counterpart.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 350, Issue 1, 25 April 2005, Pages 41-50
Journal: Gene - Volume 350, Issue 1, 25 April 2005, Pages 41-50
نویسندگان
Yu-Jin Hao, Zilian Zhang, Hiroyasu Kitashiba, Chikako Honda, Benjamin Ubi, Masayuki Kita, Takaya Moriguchi,