کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127167 | 1569975 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and characterization of gene sequences expressed in wheat spikelets at the heading stage
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
mRNATFL1TriticumAPETALA2APETALA3AP3HBPAP2PEBPANTRT-PCRmessenger RNA - RNA messengerSpike - اسپایکAINTEGUMENTA - بازپرداختGene expression - بیان ژنDifferential analysis - تجزیه و تحلیل دیفرانسیلFlowering locus T - تلقیح توت Tbase pair - جفت پایهReverse transcriptase-polymerase chain reaction - واکنش زنجیره ای واکنش زنجیره ای واکنش زنجیره ایPhosphatidylethanolamine-binding protein - پروتئین اتصال دهنده فسفاتیدیلتانولامینPISTILLATA - پسته، pista LATAFlowering - گل زدن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Through differential analysis of transcripts (SDDM), 85 cDNA sequences specifically or preferentially expressed in wheat spikelets at heading time were identified and cloned; 54 of them had significant homology with genomic, cDNA and protein and 16 with EST sequences. Among these 54 clones, 44 matched genes with known functions, whereas 10 detected homology with putative genes encoding proteins whose functions have been deduced on the basis of bioinformatic comparisons. Seventeen clones corresponded to genes that had never been cloned in cereals, 5 were related to wheat genes with known functions, and the remaining 32 to genes cloned in other cereals. On the basis of their presumed functions, the 54 clones were assigned to seven groups. The first four of them contained 40 sequences likely involved in floral organ morphogenesis and gametogenesis, and precisely (i) sequences involved in the morphogenesis of floral organs; (ii) sequences expressed in pollen and/or anther tissues; (iii) sequences encoding transcription factors; (iv) sequences involved in signal perception and transduction (kinases and LRR proteins). The expression patterns of these 40 sequences have been studied by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis of transcripts from different tissues and spike organs of wheat.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 346, 14 February 2005, Pages 221-230
Journal: Gene - Volume 346, 14 February 2005, Pages 221-230
نویسندگان
Mario Ciaffi, Anna R. Paolacci, Elisa D'Aloisio, Oronzo A. Tanzarella, Enrico Porceddu,