کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127186 | 1569974 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Purα activates PDGF-A gene transcription via interactions with a G-rich, single-stranded region of the promoter
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کلمات کلیدی
FBSEMSAPurαMBPMEFGSTNHERT-PCRPDGFnAChRβ-galElectrophoretic mobility shift assay - آزمون تحرک تحرک الکتروفورزβ-galactosidase - بتا گالاکتوزیدازTranscription - رونویسیfetal bovine serum - سرم جنین گاوplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتmouse embryo fibroblast - فیبروبلاست جنین موشKnockout mice - موش نابود شدهreal-time polymerase chain reaction - واکنش زنجیره ای پلیمراز واقعی در زمان واقعیMyelin basic protein - پروتئین پایه میلینPromoter - پروموترglutathione S-transferase - گلوتاتیون S-ترانسفرازNeuronal nicotinic acetylcholine receptor - گیرنده استیل کولین نیکوتینیک عصبی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Purα activates PDGF-A gene transcription via interactions with a G-rich, single-stranded region of the promoter Purα activates PDGF-A gene transcription via interactions with a G-rich, single-stranded region of the promoter](/preview/png/9127186.png)
چکیده انگلیسی
Transcription of the PDGF-A chain gene is regulated by multiple promoter and silencer elements that are GC-rich and exhibit considerable single-stranded character. In this study, the 42 kDa single-stranded DNA and RNA binding protein, Purα, was investigated with respect to its ability to bind and interact functionally with single-stranded DNA elements in the PDGF-A gene. Recombinant GST-Purα bound with high affinity and sequence-specificity to the G-rich strands of two such transcriptional control elements, the 5â²-S1 nuclease-hypersensitive silencer (5â²SHS; â1418 to â1388) and the nuclease-hypersensitive element (NHE; â92 to â48). Ethylation interference footprinting localized binding of Purα to a region between nucleotides â91 and â77 within the NHE element, which contains binding sites for the double-stranded DNA-binding transcription factors Sp1, EGR-1 and WT1. Forced expression of Purα upregulated transcriptional activity of the PDGF-A promoter but not the 5â²SHS silencer in HepG2 cells, demonstrating Purα has the potential to activate PDGF-A gene expression. Targeted disruption of the Purα gene reduced NHE activity and PDGF-A mRNA expression in mouse embryo fibroblasts, consistent with a physiological role for Purα in maintaining optimal transcription of the PDGF-A gene. These results indicate Purα enhances transcription of the PDGF-A gene through its interactions with single-stranded, G-rich strands in the promoter, perhaps by stabilizing non-B-form DNA conformations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 348, 28 March 2005, Pages 25-32
Journal: Gene - Volume 348, 28 March 2005, Pages 25-32
نویسندگان
Qingbei Zhang, Nancy Pedigo, Satyendra Shenoy, Kamel Khalili, David M. Kaetzel,