کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127195 | 1569974 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and characterization of the Bactrocera oleae genes orthologous to the sex determining Sex-lethal and doublesex genes of Drosophila melanogaster
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کلمات کلیدی
RRMORFTephritidaeRT-PCRGSSRBDSITcDNA - cDNAComplementary DNA - DNA تکمیلیRNA recognition motif - RNA تشخیص موتیفSex determination - تعیین جنسیتSterile insect technique - تکنیک حشرات استریلOligomerization domain - دامنه OligomerizationRNA binding domain - دامنه اتصال RNATranscription factor - عامل رونویسیopen reading frame - قاب خواندن بازpre - قبل ازreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسRNA binding protein - پروتئین متصل به RNA
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Here we report the isolation and characterization of the olive fruit fly Bactrocera oleae genes orthologous to the Drosophila melanogaster sex-determining genes Sex-lethal (Sxl) and doublesex (dsx). Fragments of the Sxl and dsx orthologous were isolated with RT-PCR. Genomic and cDNA clones were then obtained by screening a genomic library and separate male and female cDNA adult libraries using the RT-PCR products as probes in both cases. B. oleae Sxl gene (BoSxl) expresses the same pattern of transcripts which encode for a single common polypeptide in both male and female flies. The gene shares a high degree of similarity in sequence and expression to its Ceratitis capitata orthologous and does not appear to play a key regulatory role in the sex-determining cascade. B. oleae dsx gene (Bodsx) expands in a chromosomal region of more than 50 kb, with 6 exons-5 introns, producing different sex-specific mRNAs, according to the Drosophila model. The cDNA sequences are almost identical to the gene orthologous of Bactrocera tryoni. Four repeat elements identical to the D. melanogaster TRA/TRA-2 binding sites have been found in the untranslated region of the female-specific exon 4, predicting a common regulatory splicing mechanism in all studied species of Diptera.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 348, 28 March 2005, Pages 111-121
Journal: Gene - Volume 348, 28 March 2005, Pages 111-121
نویسندگان
Dimitrios Lagos, M. Fernanda Ruiz, Lucas Sánchez, Katia Komitopoulou,