کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9127198 | 1569974 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning and characterization of two novel truncated isoforms of human Na+/Ca2+ exchanger 3, expressed in fetal brain
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
SDSORF0.15 M NaCl/0.015 M Na3 citrate pH 7.6SSCUTRkilobase(s) or 1000 bpEtdBrDD-PCRXIPMTNG3PDHNCXdNTPcDNA - cDNADNA complementary to RNA - DNA مکمل RNANa+/Ca2+ exchanger - Na + / Ca2 + مبدلamino acid(s) - آمینو اسیدهاethidium bromide - اتیدیوم برومایدEST - استrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدExpressed Sequence Tag - تکرار اظهار نظرAlternative splicing - جابجایی جایگزینBase pair(s) - جفت پایه (ها)Trans-membrane domain - دامنه غشاییdeoxyribonucleoside triphosphate - دز اکسید ریبونوکلئوزید تری فسفاتCNS - دستگاه عصبی مرکزیsodium dodecyl sulfate - سدیم دودسیل سولفاتNon-redundant - غیر انحصاریopen reading frame - قاب خواندن بازRace - مسابقهuntranslated region(s) - ناحیه (های) غیر مترادفunit(s) - واحد (ها)glyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The human gene encoding the Na+/Ca2+ exchanger family member 3 (NCX3) undergoes extensive alternative splicing, with four variants previously identified. In this study, we report two novel alternative transcripts encoding two N-terminally truncated NCX3 proteins specifically expressed in human fetal brain. The identified transcripts, designated NCX3-tN.1 and NCX3-tN.2, are approximately 2.8 kb and 2.9 kb, respectively. The open reading frames (ORFs) are predicted to encode separately a 284 and a 298 amino acid (aa) polypeptide. Sequence analysis and bioinformatics reveal that NCX3-tN.1 and NCX3-tN.2 are the result of alternative splicing of the NCX3 gene. They have their own potential start codons and unique 5â² untranslated regions (UTRs) that are different from those of the known NCX3 variants. The variants include a part of intron 2 of the original gene organization as their first exon (exon “a”) at the 5â² end of the novel transcripts. NCX3-tN.2 consists of six exons including exon “a” and exons 4, 6, 7, 8 and 9 of NCX3, while NCX3-tN.1 lacks exon 4, but is otherwise similar to NCX3-tN.2. Expression studies show that both variants can be translated into protein and NCX3-tN.1 seems more efficiently translated. Based on their structural features, NCX3-tN.1 and NCX3-tN.2 proteins are potentially involved in regulation of Na+/Ca2+ homeostasis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene - Volume 348, 28 March 2005, Pages 143-155
Journal: Gene - Volume 348, 28 March 2005, Pages 143-155
نویسندگان
Rose-Marie Lindgren, Jian Zhao, Susanne Heller, Hanna Berglind, Monica Nistér,