کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9133960 | 1161664 | 2005 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An antisense RNA plays a central role in the replication control of a repC plasmid
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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![عکس صفحه اول مقاله: An antisense RNA plays a central role in the replication control of a repC plasmid An antisense RNA plays a central role in the replication control of a repC plasmid](/preview/png/9133960.png)
چکیده انگلیسی
The widespread replicons of repABC and repC families from α-proteobacteria share high similarity in their replication initiator proteins (RepC). Here we describe the minimal region required for stable replication of a member of the repC family, the low copy-number plasmid pRmeGR4a from Sinorizobium meliloti GR4. This region contains only two genes: one encoding the initiator protein RepC (46.8 kDa) and other, an antisense RNA (67 nt). Mapping of transcriptional start sites and promoter regions of both genes showed that the antisense RNA is nested within the repC mRNA leader. The constitutively expressed countertranscribed RNA (ctRNA) forms a single stem-loop structure that acts as an intrinsic rho-independent terminator. The ctRNA is a strong trans-incompatibility factor and negative regulator of repC expression. Based on structural and functional similarities between members of the repC and repABC families we propose a model of their evolutionary relationship.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 54, Issue 3, November 2005, Pages 259-277
Journal: Plasmid - Volume 54, Issue 3, November 2005, Pages 259-277
نویسندگان
Javier Izquierdo, Tatiana Venkova-Canova, Miguel A. RamÃrez-Romero, Juan Téllez-Sosa, Ismael Hernández-Lucas, Juan Sanjuan, Miguel A. Cevallos,