کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9133962 1161664 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electroporation-mediated transformation of Aeromonas hydrophila
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Electroporation-mediated transformation of Aeromonas hydrophila
چکیده انگلیسی
A strain of Aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as PHBHHx, was successfully transformed by electroporation. The plasmid used was a broad host range plasmid pBBR1MCS. Electroporation conditions were varied systemically to develop an electroporation protocol. The optimal yield of transformant was approximately 4 × 102 CFU/μg DNA at 12.5 kV/cm and 1000 Ω, resulting in a time constant of approximately 5 ms. The A. hydrophila transformants expressed plasmid-encoded resistance to chloromphenicol. Plasmid DNA in the A. hydrophila transformant was stably maintained. This is the first report of transformation of bacteria A. hydrophila.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 54, Issue 3, November 2005, Pages 283-287
نویسندگان
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