کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9133998 1161672 2005 16 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Limited use of the Cre/loxP recombination system in efficient production of loxP-containing minicircles in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Limited use of the Cre/loxP recombination system in efficient production of loxP-containing minicircles in vivo
چکیده انگلیسی
The Cre/loxP recombination system of bacteriophage P1 is one of the most powerful tools in genome engineering. We report, however, that the activity of the Cre/loxP system interferes with the stability of the multicopy loxP-bearing plasmids in Escherichia coli recA bacteria. Due to the predominantly unidirectional Cre-mediated high-order multimer formation of these plasmids, the number of their copies (overall yield) gradually decreases. Intermolecular recombination reduces the copy number of plasmids and eventually increases their segregational instability. We have found that in the presence of even the slightest amount of Cre activity, loxP-bearing plasmids continuously undergo multimerization, which very rapidly leads to loxP-plasmid free cells. Our results are compatible with the hypothesis of the multimer catastrophe [Cell, 1984 (36), 1097].
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 53, Issue 2, March 2005, Pages 148-163
نویسندگان
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