کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9134031 | 1161675 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TnXO1, a germination-associated class II transposon from Bacillus anthracis
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
ژنتیک
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چکیده انگلیسی
Bacillus anthracis harbours two virulence plasmids, pXO1 (182Â kb) and pXO2 (95Â kb). Whereas pXO2 harbours the cap operon coding for the capsule, pXO1 contains the pag, lef, and cya genes coding for protective antigen, lethal, and oedema factors, respectively, as well as the atxA regulatory gene. These genes are located within a 44.8Â kb long pathogenicity island flanked by insertion sequences. Here, we describe the presence in the same plasmid region of an 8679Â bp genetic element displaying the structural features of a class II cointegrative transposon. This element, named TnXO1, bears a transposase and a site-specific recombinase and is delineated by 38Â bp terminal inverted repeats sequences similar to those of other members of this group of transposons. A putative res site has been identified in the 200Â bp region between these genes. Interestingly, TnXO1 also contains the gerX operon involved in the germination of B. anthracis spores within phagocytic cells. Such close association of a mobile DNA structure with known virulence determinants in a pathogen further prompted us to look for the presence of this transposable element in other members of the Bacillus cereus sensu lato group. No instance of TnXO1 was detected outside of B. anthracis in PCR experiments, although it was found to be present in the genome sequence draft of one strain of B. cereus which has recently been shown to harbour a plasmid almost identical to pXO1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 53, Issue 3, May 2005, Pages 251-257
Journal: Plasmid - Volume 53, Issue 3, May 2005, Pages 251-257
نویسندگان
Géraldine Van der Auwera, Jacques Mahillon,