کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9138898 | 1162822 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima Native crystal structure of a nitric oxide-releasing lectin from the seeds of Canavalia maritima](/preview/png/9138898.png)
چکیده انگلیسی
Here, we report the crystallographic study of a lectin from Canavalia maritima seeds (ConM) and its relaxant activity on vascular smooth muscle, to provide new insights into the understanding of structure/function relationships of this class of proteins. ConM was crystallized and its structure determined by standard molecular replacement techniques. The amino acid residues, previously suggested incorrectly by manual sequencing, have now been determined as I17, I53, S129, S134, G144, S164, P165, S187, V190, S169, T196, and S202. Analysis of the structure indicated a dimer in the asymmetric unit, two metal binding sites per monomer, and loops involved in the molecular oligomerization. These confer 98% similarity between ConM and other previously described lectins, derived from Canavalia ensiformis and Canavalia brasiliensis. Our functional data indicate that ConM exerts a concentration-dependent relaxant action on isolated aortic rings that probably occurs via an interaction with a specific lectin-binding site on the endothelium, resulting in a release of nitric oxide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 152, Issue 3, December 2005, Pages 185-194
Journal: Journal of Structural Biology - Volume 152, Issue 3, December 2005, Pages 185-194
نویسندگان
Carlos Alberto de Almeida Gadelha, Frederico Bruno Mendes Batista Moreno, Tatiane Santi-Gadelha, João Batista Cajazeiras, Bruno Anderson Matias da Rocha, Ana Maria Sampaio Assreuy, Mário Rogério Lima Mota, Nilson Vieira Pinto,