کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9139129 | 1162836 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-throughput film-densitometry: an efficient approach to generate large data sets
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to “near-to-perfect” performance. Due to the large area of the standard electron microscope film that can be digitized by the commercial densitometer (up to 10,000Â ÃÂ 13,680 pixels with an appropriately coded holder), automated film digitization offers a fast and inexpensive alternative to high-end CCD cameras as a means of acquiring large amounts of image data in electron microscopy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Structural Biology - Volume 149, Issue 1, January 2005, Pages 17-29
Journal: Journal of Structural Biology - Volume 149, Issue 1, January 2005, Pages 17-29
نویسندگان
Dieter Typke, Robert A. Nordmeyer, Arthur Jones, Juyoung Lee, Agustin Avila-Sakar, Kenneth H. Downing, Robert M. Glaeser,