کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9139932 | 1163385 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-level expression of the Toxoplasma gondii STT3 gene is required for suppression of the yeast STT3 gene mutation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
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چکیده انگلیسی
N-linked glycosylation is the most frequent modification of secretory proteins. The central reaction of this process in eukaryotic cells is catalyzed by the hetero-oligomeric protein complex oligosaccharyltransferase (OST). The gene STT3 gene encodes a protein, which is the most conserved among the components of the OST. In this report, we describe the isolation and functional characterization of a STT3 homologue from Toxoplasma gondii. The topology of the TgStt3p is similar to that of the yeast Stt3p with 47% identity. We demonstrate that high level expression of the homologues gene is required to completely suppress the defect caused by a stt3 mutation in yeast, suggesting that homologous Stt3 proteins can serve analogous functions in distantly related eukaryotic cells regardless of their degree of conservation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 143, Issue 1, September 2005, Pages 6-11
Journal: Molecular and Biochemical Parasitology - Volume 143, Issue 1, September 2005, Pages 6-11
نویسندگان
Hosam Shams-Eldin, Thomas Blaschke, Darisuren Anhlan, Sebastian Niehus, Jan Müller, Nahid Azzouz, Ralph T. Schwarz,