کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9140000 | 1163390 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding affinity and capacity of putative adaptor-mediated sorting of a Type I membrane protein in Leishmania mexicana
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Binding affinity and capacity of putative adaptor-mediated sorting of a Type I membrane protein in Leishmania mexicana Binding affinity and capacity of putative adaptor-mediated sorting of a Type I membrane protein in Leishmania mexicana](/preview/png/9140000.png)
چکیده انگلیسی
The membrane-bound acid phosphatase (MBAP), a Type I membrane protein predominantly associated with endosomal/lysosomal structures of Leishmania mexicana promastigotes, contains motifs in its cytosolic COOH-terminal tail (-MEVWRRYMKFKNKQSEAIIV-COOH) akin to tyrosine- and di-leucine-based sorting signals in multicellular organisms. Here, we first show that the COOH-terminal residues IIV of MBAP, but not the Y-residue, are required for endosomal targeting, suggesting specific binding to an adaptor complex at the cell surface. We then determine whether specific binding can be saturated by analysing the efficiency of endosomal targeting for increasing numbers of MBAP molecules per cell. The ratio of the steady-state abundance of wild-type MBAP on the cell surface to MBAP on endosomes increases until the distribution is no longer different from that observed for a mutant MBAP which lacks the IIV-motif or for a glycosylphosphatidylinositol-anchored form, both of which are distributed according to bulk membrane flow. A quantitative analysis of these in vivo results indicates specific binding to a putative adaptor complex with an affinity of about 10â4Â M to 50,000 sorting sites on the cell surface.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Biochemical Parasitology - Volume 142, Issue 2, August 2005, Pages 203-211
Journal: Molecular and Biochemical Parasitology - Volume 142, Issue 2, August 2005, Pages 203-211
نویسندگان
Frank Weise, Lutz Thilo, Markus Engstler, Martin Wiese, Isabel Benzel, Christina Kühn, Hans-Jörg Bühring, Peter Overath,