کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9141679 | 1163865 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of L-type calcium channel splice variants expressed in human T lymphocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PBTs12-O-tetradecanoylphorbol 13-acetateDHPTRPMTrpTRPVICRACperipheral blood T lymphocytesRT-PCRGAPDHtPA1,4-Dihydropyridine - 1،4-دی هیدروپیریدینCa2+ - Ca2 +[Ca2+]i - [Ca2 +] ino treatment - بدون درمانrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدintracellular Ca2+ concentration - غلظت Ca2 + داخل سلولیCellular activation - فعال سازی تلفن همراهHuman T lymphocytes - لنفوسیت T انسانRace - مسابقهtransient receptor potential - پتانسیل گیرنده گذراCalcium channel - کانال کلسیمCalcium - کلسیمglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی مولکولی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization of L-type calcium channel splice variants expressed in human T lymphocytes Molecular characterization of L-type calcium channel splice variants expressed in human T lymphocytes](/preview/png/9141679.png)
چکیده انگلیسی
Calcium (Ca2+) influx is a fundamental intracellular signal that is required to initiate and sustain T lymphocyte activation. Dihydropyridine-sensitive, L-type Ca2+ channels appear to play a significant role in Ca2+ mobilization during T cell activation, but very little is known about the molecular structure of these channels in T lymphocytes. Here we identify two novel splice variants of the CaV1.4 (α1F) L-type Ca2+ channel that are expressed in human T lymphocytes, and also demonstrate expression of the CaV1.4 protein in the human Jurkat T cell leukemia line and human peripheral blood T lymphocytes (PBTs). The carboxy-termini of both CaV1.4 splice isoforms contain unique exon usages distinct from the CaV1.4 channel isolated from human retina that may render these channel variants insensitive to changes in membrane depolarization. Additional evidence of the importance of these new splice variants comes from the demonstration that the mRNA expression of the CaV1.4 splice isoforms is regulated by TCR-induced activation in Jurkat T cells, and to a lesser extent in human PBTs. Overall these results provide the first evidence that structurally unique L-type Ca2+ channels exist in T lymphocytes, which can contribute to a Ca2+ influx during T lymphocyte activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 42, Issue 12, August 2005, Pages 1461-1474
Journal: Molecular Immunology - Volume 42, Issue 12, August 2005, Pages 1461-1474
نویسندگان
Maya F. Kotturi, Wilfred A. Jefferies,