کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9144608 | 1164523 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and analysis of candidate ascochyta blight defence genes in chickpea. Part II. Microarray expression analysis of putative defence-related ESTs
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Using microarray technology and previously identified defence-related ESTs from chickpea (Cicer arietinum L.), the ascochyta blight (Ascochyta rabiei (Pass.) Labrousse) resistance response was studied in a highly resistant chickpea accession (ICC3996) and a susceptible cultivar (Lasseter). The time-series expression patterns of 20 defence-related ESTs were studied after inoculation with A. rabiei. Ten of the defence-related ESTs displayed up- or down-regulation in ICC3996 and/or Lasseter compared to uninoculated control samples. Hierarchical clustering grouped the ESTs into clusters of similar observations, revealing that three defence related ESTs showed differential up-regulation in ICC3996 when compared to Lasseter-a leucine zipper protein, SNAKIN2 antimicrobial peptide precursor, and elicitor-induced receptor protein. The potential involvement of these ESTs in effective chickpea defence against ascochyta blight is discussed. The microarray expression analysis of defence-related ESTs in chickpea represents the first study of this type, and future work will focus on large-scale expression studies aimed at further elucidating the function of genes involved in ascochyta blight resistance and the pathway of their action.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physiological and Molecular Plant Pathology - Volume 66, Issue 5, May 2005, Pages 201-210
Journal: Physiological and Molecular Plant Pathology - Volume 66, Issue 5, May 2005, Pages 201-210
نویسندگان
Tristan E. Coram, Edwin C.K. Pang,