Antecubital vein venous sampling does not distort circulating levels of peptide and sex steroid hormones

Simultaneous sampling from the antecubital vein (ACV) and a heated superficial hand vein (HSHV) at 10-minute intervals was performed in 6 women for determination of glucose, insulin, luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin, estradiol (E2), progesterone (P4), testosterone (T), and dehydroepiandrosterone sulfate (DHEAS). Strong correlations were identified for each parameter between the two vessels, with luteinizing hormone (LH) and follicle-stimulating hormone (FSH) pulse patterns nearly superimposable between antecubital vein (ACV) and superficial hand vein (HSHV) samples.