کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9335292 | 1256876 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Influence of embryonic conditioned media upon the endometrial β-adrenergic receptor
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موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
زنان، زایمان و بهداشت زنان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The main objective of this study was to evaluate the influence of the products secreted by the human embryo upon the three subtypes of β-AR (β1, β2, β3). Cell cultures were developed using endometrial biopsies, taken on day 7 after ovulation, from four healthy women <35 years of age, with regular cycles and infertility due only to male factors. Embryos from women with a normal uterine cavity and endometrial lining were incubated until they reached the 4-cell stage, before being transferred to their mother's uterus. Culture media for embryo incubation were derived from two groups: (i) embryos that achieved pregnancy, (ii) embryos which failed to implant. Control and experimental endometrial cell culture plates were treated with the two embryo culture media, with or without 10â9 mol/l oestradiol and 10â7 mol/l progesterone for 48 h. Expression of the three subtypes of β-AR was assessed by RT-PCR. β1-AR was expressed in both control and experimental plates; β2-AR was expressed only in plates incubated with embryonic culture media of embryos which achieved pregnancy, in both hormonal conditions, with or without oestradiol and progesterone. β3-AR was not expressed in any condition. Thus secretory products of human embryos may influence gene expression of β2-AR concentrations in the human endometrium, and this subtype of β-AR may be involved in implantation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive BioMedicine Online - Volume 11, Issue 1, 2005, Pages 58-63
Journal: Reproductive BioMedicine Online - Volume 11, Issue 1, 2005, Pages 58-63
نویسندگان
ME Bruzzone, C Fabres, DA Benitez, EA Castellón, F Zegers-Hochschild,