کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9415703 | 1614321 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Geranylgeranylacetone, a heat shock protein inducer, prevents acoustic injury in the guinea pig
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کلمات کلیدی
PBSJnkGeranylgeranylacetoneHspABRc-Jun N terminal kinaseHSF1nNOSHSPsGGA - ARFNoise trauma - آسیب سر و صداIHC - ایمونوهیستوشیمیInner hair cell - سلول داخلی داخلیHair cells - سلول های مویneuronal nitric oxide synthase - سنتاز اکسید نیتریک عصبیHearing - شنیدن یا شنواییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریauditory brainstem response - پاسخ شنوایی مغزHeat shock protein - پروتئین شوک حرارتHeat shock proteins - پروتئینهای شوک حرارتیInner ear - گوش داخلیAuditory, vestibular, and lateral line: periphery - گوش شنوایی، وستیبولی و لبه جانبی: محدوده
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Geranylgeranylacetone (GGA) used widely as anti-ulcer agent is accepted as an inducer of the heat shock proteins (Hsps) at gastric mucosa, liver, heart, and brain. However, there have been no reports that GGA could induce Hsps in the cochlea leading up to the oto-protection. The purpose of the present study was to investigate whether single oral dose of GGA could induce Hsps at cochlea and oral administration had protective effect to the cochlea against noise trauma. We used Hartley guinea pigs and investigated the expression of Hsp70, 40, and 27 in cochlea by Western blot analysis. To evaluate cochlear function, we assessed thresholds of the auditory brain stem response (ABR). For histological assessment, we observed the sensory epithelium using surface preparation technique. GGA (600 mg/kg) or vehicle was given orally to animals. Western blot analysis showed that the expressions of Hsp 70, 40, and 27 were increased 24-48 h after administration of single dose of GGA, whereas there was less expression in the animals given vehicle. In the animals given GGA once a day for a week before sound exposure (130 dB SPL octave band noise with a center frequency of 4 kHz) for 3 h, their ABR threshold elevations were lowered significantly. In addition, significantly fewer defects were observed on outer hair cells of organ of Corti in the animals treated by GGA than those of the animals without GGA. This result shows that pretreatment by GGA have a potential to prevent cochlea damage against the intense noise.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1065, Issues 1â2, 14 December 2005, Pages 107-114
Journal: Brain Research - Volume 1065, Issues 1â2, 14 December 2005, Pages 107-114
نویسندگان
Takefumi Mikuriya, Kazuma Sugahara, Tsuyoshi Takemoto, Kuniyoshi Tanaka, Kenji Takeno, Hiroaki Shimogori, Akira Nakai, Hiroshi Yamashita,