Adenosine A1 receptors mediate inhibition of cAMP formation in vitro in the pontine, REM sleep induction zone

Microinjection of adenosine A1 receptor agonist or an inhibitor of adenylyl cyclase into the caudal, oral pontine reticular formation (PnOc) of the rat induces a long-lasting increase in REM sleep. Here, we report significant inhibition of forskolin-stimulated cAMP in dissected pontine tissue slices containing the PnOc incubated with the A1 receptor agonist, cyclohexaladenosine (10−8 M). These data are consistent with adenosine A1 receptor agonist actions on REM sleep mediated through inhibition of cAMP.