کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9422707 | 1294695 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of GABA and glutamate in vivo levels with high sensitivity and frequency
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Measurement of GABA and glutamate in vivo levels with high sensitivity and frequency Measurement of GABA and glutamate in vivo levels with high sensitivity and frequency](/preview/png/9422707.png)
چکیده انگلیسی
In the present protocol, we demonstrate a high-performance liquid chromatography (HPLC) system that enables detection of very low amounts of γ-aminobutyric acid (GABA) (0.03 pmol) and glutamate (0.8 pmol). The HPLC system consists of two pumps, an electrochemical detector, a high-pressure six-way switching valve, a guard column, a microbore column, and a column oven. A microdialysis probe was implanted in the right parietal cortex in rats. Dialysates were collected every 5 min and were split into two equal aliquots for separate analysis of GABA and glutamate. After derivatization with o-phthalaldehyde (OPA), samples were isocratically separated and purified by the guard column. To make the peak of GABA or glutamate appear in an opportune place in a chromatogram, a six-way switching valve was used to control the eluate containing GABA or glutamate to be led to the microbore column and electrochemical detector. By the use of this system, decrease in extracellular concentration of GABA, which precedes the appearance of electrical discharge initiated by hyperbaric oxygen (HBO2) exposure, was detected by microdialysis at the time resolution of 5 min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research Protocols - Volume 14, Issue 2, February 2005, Pages 61-66
Journal: Brain Research Protocols - Volume 14, Issue 2, February 2005, Pages 61-66
نویسندگان
Shan Zhang, Yoshimasa Takeda, Shingo Hagioka, Ken Takata, Hisami Aoe, Hideki Nakatsuka, Masataka Yokoyama, Kiyoshi Morita,