کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9424340 | 1295261 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
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چکیده انگلیسی
There is increasing evidence that oxidative stress plays an important role in the pathogenesis of many neurodegenerative diseases including Parkinson's disease (PD). In particular there is support for the participation of oxidized catecholamines in PD. Catecholamines are highly reactive and are readily oxidized to aminochromes. While aminochromes have been shown to be toxic, their formation in oxidative stress and subsequent participation in disease has yet to be confirmed. We propose that the characterization of aminochromes, specifically dopaminochrome, is important in clarifying the role that oxidized catecholamines play in PD. We have developed a novel method for the separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection (HPLC-ED). Our method utilizes the separation principles employed in measuring catecholamines by HPLC except that the electrochemical detection of aminochromes is achieved by reversing the detector's electrode. We have used this method to separate and quantify aminochrome standards, prepared by oxidizing catecholamines with sodium periodate (NaIO4) and we have also shown that aminochromes can be measured in plasma and cell lysates. Furthermore, we have characterized aminochromes to facilitate forthcoming studies on aminochromes and the role oxidized catecholamines may play in neurodegenerative disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Neuroscience Methods - Volume 142, Issue 2, 30 March 2005, Pages 201-208
Journal: Journal of Neuroscience Methods - Volume 142, Issue 2, 30 March 2005, Pages 201-208
نویسندگان
Scott D. Ochs, Thomas C. Westfall, Heather Macarthur,