An integrated spinal cord-hindlimbs preparation for studying the role of intrinsic properties in somatosensory information processing

Several studies performed using the slice in vitro technique have shown that spinal cord neurons display specialized intrinsic electrophysiological properties. However, the actual role of intrinsic properties in somatosensory processing remains unclear, mainly due to the impossibility to generate natural sensory inputs in spinal cord slices. Here, we show an integrated spinal cord-hindlimbs preparation of juvenile turtles that has the advantages of in vitro approaches and still enables natural stimulation. By making patch-clamp whole-cell recordings of both superficial and deep dorsal horn neurons in the integrated preparation, we found similar electrophysiological phenotypes as those observed in slices. Most of the cells responded to natural stimuli, had large receptive fields and were classified as wide-dynamic range neurons. Both low-threshold spikes and plateau potentials interacted with naturally evoked sensory inputs, generating complex dynamics. Furthermore, we found that the activity of the spinal cord network induced by natural stimulation modulated the excitability of plateau-generating cells. This effect was mimicked by bath application of cis-(±)-1-aminocyclopentane-1,3-dicarboxylic acid (ACPD), a group I glutamate metabotropic receptor agonist. Our results show that the spinal cord-hindlimbs preparation represents a valuable model to study the contribution of intrinsic properties to early stages of somatosensory information processing.