کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425430 | 1295872 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adenosine A1 and A2A receptors are co-expressed in pyramidal neurons and co-localized in glutamatergic nerve terminals of the rat hippocampus
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کلمات کلیدی
PBSGFAPVGLUTBSA - BSAbovine serum albumin - آلبومین سرم گاوCo-expression - بیان مشترکbase pairs - جفت پایهvesicular glutamate transporter - حمل و نقل گلوتامات vesicularreverse transcription - رونویسی معکوسSynaptosomes - سیناپتوزوم هاPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCo-localization - محلی سازیGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالglutamate - گلوتامات
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adenosine is a neuromodulator that controls neurotransmitter release through inhibitory A1 and facilitatory A2A receptors. Although both adenosine receptor-mediated inhibition and facilitation of glutamate release have been observed, it is not clear whether both A1 and A2A receptors are located in the same glutamatergic nerve terminal or whether they are located on different populations of these terminals. Thus, we have tested if single pyramidal glutamatergic neurons from the hippocampus simultaneously expressed A1 and A2A receptor mRNA and if A1 and A2A receptors were co-localized in hippocampal glutamatergic nerve terminals. Single cell PCR analysis of visually identified pyramidal neurons revealed the simultaneous presence of A1 and A2A receptor mRNA in four out 16 pyramidal cells possessing glutamatergic markers but not GABAergic or astrocytic markers. Also, A1 and A2A receptor immunoreactivities were co-localized in 26±4% of nerve terminals labeled with antibodies against vesicular glutamate transporters type 1 or 2, i.e. glutamatergic nerve terminals. This indicates that glutamatergic neurons in the hippocampus co-express A1 and A2A receptors and that these two receptors are co-localized in a subset of glutamatergic nerve terminals.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 133, Issue 1, 2005, Pages 79-83
Journal: Neuroscience - Volume 133, Issue 1, 2005, Pages 79-83
نویسندگان
N. Rebola, R.J. Rodrigues, L.V. Lopes, P.J. Richardson, C.R. Oliveira, R.A. Cunha,