کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9425886 | 1295896 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of Chicken Transmembrane Channel-like (TMC) genes: Expression analysis in the cochlea
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کلمات کلیدی
PBSGAPDHSCApreimmuneVestibularTMCEST - استTonotopic - تنوتوبیکExpressed Sequence Tag - تکرار اظهار نظرhair cell - سلول موHearing - شنیدن یا شنواییtegmentum vasculosum - عصبانیتtransmembrane - فرابنفشPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریmegabase - مگابایتیbasilar papilla - پاپیلار اساسیglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژناز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of Chicken Transmembrane Channel-like (TMC) genes: Expression analysis in the cochlea Identification of Chicken Transmembrane Channel-like (TMC) genes: Expression analysis in the cochlea](/preview/png/9425886.png)
چکیده انگلیسی
Mutations of the human gene encoding transmembrane channel-like protein (TMC)1 cause dominant and recessive nonsyndromic hearing disorders, suggesting that this protein plays an important role in the inner ear. In this study, we cloned chicken Tmc2 (GgTmc2) from a cochlear cDNA library and we annotated four additional TMC family members: GgTmc1, GgTmc3, GgTmc6, and GgTmc7. All chicken TMCs possess the defining TMC signature motif and display high conservation of their genomic structure when compared with other vertebrate TMC genes. GgTmc1 is localized on the chicken sex chromosome Z at a locus that displays conserved synteny with the loci of mammalian orthologues residing on autosomes. In contrast, the locus of GgTmc2 does not exhibit conserved synteny with its mammalian orthologues. Because murine TMC1 and TMC2 are restrictively expressed in cochlear hair cells, we determined the expression of the chicken orthologues in the basilar papilla, the avian equivalent of the organ of Corti. While GgTmc2 was present throughout the basilar papilla and in other tissues, GgTmc1 transcript was detected specifically in the basal portion of the basilar papilla and was not detectable in any other tissue or organ studied. GgTmc3 and GgTmc6 were detectable in all organs analyzed. Antibody labeling revealed that GgTmc2 is predominantly associated with the lateral membranes of hair and supporting cells. The expression of GgTmc2 by both cell types was further confirmed by RT-PCR using isolated cells. This expression and subcellular localization of GgTmc2 is in agreement with the proposed potential role of this novel class of transmembrane proteins in ion transport.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 132, Issue 4, 2005, Pages 1115-1122
Journal: Neuroscience - Volume 132, Issue 4, 2005, Pages 1115-1122
نویسندگان
H. Mutai, S. Mann, S. Heller,