کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9426713 | 1295932 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deletion of presynaptic adenosine A1 receptors impairs the recovery of synaptic transmission after hypoxia
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کلمات کلیدی
CPTCA1GFPaCSFAAV8-cyclopentyltheophylline - 8-سیکلوپنتیل تئوفیلینDMSO - DMSOfEPSPs - fEPSP هاElectrophysiology - الکتروفیزیولوژیDimethyl sulfoxide - دیمتیل سولفواکسیدSchaffer collaterals - شواهدartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیAdeno-associated virus - ویروس Adeno مرتبط استfield excitatory postsynaptic potentials - پتانسیل پستنیپتیک تحریک پذیری میدانgreen fluorescent protein - پروتئین فلورسنت سبزCre recombinase - کرم recombinase
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Adenosine protects neurons during hypoxia by inhibiting excitatory synaptic transmission and preventing NMDA receptor activation. Using an adeno-associated viral (AAV) vector containing Cre recombinase, we have focally deleted adenosine A1 receptors in specific hippocampal regions of adult mice. Recently, we found that deletion of A1 receptors in the CA1 area blocks the postsynaptic responses to adenosine in CA1 pyramidal neurons, and deletion of A1 receptors in CA3 neurons abolishes the presynaptic effects of adenosine on the Schaffer collateral input [J Neurosci 23 (2003) 5762]. In the current study, we used this technique to delete A1 receptors focally from CA3 neurons to investigate whether presynaptic A1 receptors protect synaptic transmission from hypoxia. We studied the effects of prolonged (1 h) hypoxia on the evoked field excitatory postsynaptic potentials (fEPSPs) in the CA1 region using in vitro slices. Focal deletion of the presynaptic A1 receptors on the Schaffer collateral input slowed the depression of the fEPSPs in response to hypoxia and impaired the recovery of the fEPSPs after hypoxia. Delayed responses to hypoxia linearly correlated with impaired recovery. These findings provide direct evidence that the neuroprotective role of adenosine during hypoxia depends on the rapid inhibition of synaptic transmission by the activation of presynaptic A1 receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience - Volume 132, Issue 3, 2005, Pages 575-580
Journal: Neuroscience - Volume 132, Issue 3, 2005, Pages 575-580
نویسندگان
E. Arrigoni, A.J. Crocker, C.B. Saper, R.W. Greene, T.E. Scammell,