کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9434444 | 1298153 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and functional expression of the bovine GABAC Ï2 subunit
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cloning and functional expression of the bovine GABAC Ï2 subunit Cloning and functional expression of the bovine GABAC Ï2 subunit](/preview/png/9434444.png)
چکیده انگلیسی
In this study, using RT-PCR, we found that the Ï1 and Ï2 subunits are widely expressed in the CNS including areas where they have not been previously described such as the bulb, pons and the caudate nucleus. To determine if the GABAC component of the GABA-currents elicited by oocytes expressing cerebellum mRNA was caused by activation of homomeric GABA Ï2 receptors, we cloned the corresponding cDNA and expressed it in Xenopus oocytes. It was found that oocytes injected with Ï2 cDNA, efficiently formed GABA-gated homooligomeric receptors. The GABA-dose-current response gave an EC50 = 1.19 μM and the currents were resistant to bicuculline and reversibly antagonized by the specific GABAC receptor antagonist TPMPA. Altogether, our results indicate a widespread distribution of both Ï1 and Ï2 subunits in the bovine CNS and show further that the Ï2 subunit cDNA isolated from cerebellum, forms fully functional receptors when expressed in Xenopus oocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Research - Volume 53, Issue 4, December 2005, Pages 421-427
Journal: Neuroscience Research - Volume 53, Issue 4, December 2005, Pages 421-427
نویسندگان
Ariel López-Chávez, Ricardo Miledi, Ataúlfo MartÃnez-Torres,