کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9475164 | 1322874 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transformation of chrysanthemum with mutated ethylene receptor genes: mDG-ERS1 transgenes conferring reduced ethylene sensitivity and characterization of the transformants
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
We generated mutated ethylene receptor genes (mDG-ERS1s) from the chrysanthemum ethylene receptor (DG-ERS1) cDNA by introducing one-nucleotide substitutions corresponding to those present in Arabidopsis etr1-1, etr1-2, etr1-3, and etr1-4 and tomato Nr. The promoter of a tobacco elongation factor 1α (EF1α) gene was fused to DG-ERS1 cDNA or one of the mDG-ERS1s. The resulting constructs were named EF1αâ·mDG-ERS1(etr1-1), -ERS1(etr1-2) and so on, and introduced into chrysanthemum cv. Sei-Marine. We obtained putative transformants resistant to an antibiotic paromomycin with a yield of 2.4-6.2% depending on the construct. The mDG-ERS1(etr1-4) construct tended to be more effective in conferring reduced ethylene sensitivity in chrysanthemum than the others. PCR analysis gave amplification corresponding to a partial sequence of EF1αâ·mDG-ERS1 transgenes. Southern blot analysis showed that, in the mDG-ERS1(etr1-4) transformant, not only the lines with reduced sensitivity to ethylene but also those sensitive to ethylene harbored the mDG-ERS1(etr1-4) transgene. The present results showed the usefulness of mutated ethylene receptor genes mDG-ERS1s for generation of transgenic chrysanthemums with reduced ethylene sensitivity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Postharvest Biology and Technology - Volume 37, Issue 2, August 2005, Pages 101-110
Journal: Postharvest Biology and Technology - Volume 37, Issue 2, August 2005, Pages 101-110
نویسندگان
Takako Narumi, Ryutaro Aida, Akemi Ohmiya, Shigeru Satoh,