کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9475325 | 1322886 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of α-farnesene synthase gene AFS1 in relation to levels of α-farnesene and conjugated trienols in peel tissue of scald-susceptible 'Law Rome' and scald-resistant 'Idared' apple fruit
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Nontreated control and 1-MCP-treated fruit of the scald-susceptible 'Law Rome' and scald-resistant 'Idared' cultivars were compared with respect to scald incidence, internal ethylene concentration (IEC), α-farnesene metabolism, and expression of the gene encoding α-farnesene synthase (AFS1), the final, rate-limiting enzyme in the α-farnesene biosynthetic pathway. The incidence of scald in nontreated 'Law Rome' apples after 20 weeks at 0.5 °C plus 1 week at 20 °C averaged 86%; 1-MCP treatment reduced increases in IEC and reduced the incidence of scald to less than 1%. Fruit of 'Idared' showed no scald regardless of the treatment. 1-MCP also inhibited α-farnesene production, suggesting that ethylene induces transcription of key genes involved in α-farnesene biosynthesis. In both 'Law Rome' and 'Idared', a sharp increase in AFS1 mRNA during the first 4-8 weeks of storage preceded a proportional rise in α-farnesene and a subsequent increase in CTols. However, maximum levels of AFS1 transcript, α-farnesene, and CTols were, respectively, 2.5-, 4-, and 33-fold greater in 'Law Rome' than in 'Idared' apples. 1-MCP suppressed the increases in AFS1 transcript and α-farnesene early in storage, although AFS1 expression and α-farnesene synthesis had recovered in treated 'Law Rome' fruit after 20 weeks, consistent with increasing IEC in these fruit.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Postharvest Biology and Technology - Volume 35, Issue 2, February 2005, Pages 125-132
Journal: Postharvest Biology and Technology - Volume 35, Issue 2, February 2005, Pages 125-132
نویسندگان
Steven W. Pechous, Christopher B. Watkins, Bruce D. Whitaker,