کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9587425 1393241 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Photo-CIDNP NMR spectroscopy of a heme-containing protein
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی تئوریک و عملی
پیش نمایش صفحه اول مقاله
Photo-CIDNP NMR spectroscopy of a heme-containing protein
چکیده انگلیسی
There are relatively few examples of the application of photo-CIDNP NMR spectroscopy to chromophore-containing proteins. The most likely reason for this is that simultaneous absorption of light by the photosensitiser molecule and the protein chromophore reduces the effectiveness of the photochemical reaction that produces the observed nuclear polarisation. We present details of experiments performed on the air-oxidised form of a small cytochrome, from the thermophilic bacterium Hydrogenobacter thermophilus, using both the wild-type protein and apo and holo forms of a double alanine b-type mutant. We show that, along with the apo state, it is possible to generate CIDNP in the air-oxidised form of the b-type mutant, but not in the corresponding c-type cytochrome. This finding is supported by control experiments using horse-heart cytochrome c.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Magnetic Resonance - Volume 175, Issue 2, August 2005, Pages 330-335
نویسندگان
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