کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
9657 641 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Supercritical CO2-assisted embossing for studying cell behaviour on microtextured surfaces
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Supercritical CO2-assisted embossing for studying cell behaviour on microtextured surfaces
چکیده انگلیسی

Recently, cell responses to micro- and nanoscale structures have attracted much attention. Although interesting phenomena have been observed, we have encountered some difficulties in elucidating purely topographical effects on cell behaviour. These problems are partially attributable to the introduction of functional groups and the persistence of chemicals during surface processing. In this study, we introduced supercritical CO2-assisted embossing, which plasticizes a polycarbonate plate by dissolving supercritical CO2 and thus can emboss wide-scale patterns onto the plate at a lower temperature than the polycarbonate glass transition temperature. Uniform micro- and nanopatterned surfaces were observed across the whole area of the polycarbonate plate surfaces. Nickel, fluorine, and nitrogen were not detected on the fabricated surfaces, and the surface carbon-to-oxygen ratios were equivalent to the theoretical ratio (C:O = 84.2:15.8) calculated from the polycarbonate molecular structure. Human mesenchymal stem cells were cultured on the fabricated microlens and nanogroove substrata. Cell-adhered areas became smaller on the microlens than on non-treated polycarbonate. Meanwhile, cells aligned along the ridges of nanogrooves with valleys deeper than 90 nm. This supercritical CO2-assisted embossing can produce fine substrates for studying the effects of surface topography of synthetic materials on cell behaviours.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 29, Issue 34, December 2008, Pages 4494–4500
نویسندگان
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