کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9818214 | 1518777 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of cell motility on proton beam micromachined 3D scaffolds
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
سطوح، پوششها و فیلمها
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Measurement of cell motility on proton beam micromachined 3D scaffolds Measurement of cell motility on proton beam micromachined 3D scaffolds](/preview/png/9818214.png)
چکیده انگلیسی
Tissue engineering is a rapidly developing and highly interdisciplinary field that applies the principles of cell biology, engineering and material science. In natural tissues, the cells are arranged in a three-dimensional (3D) matrix which provides the appropriate functional, nutritional and spatial conditions. In scaffold guided tissue engineering 3D scaffolds provide the critical function of acting as extracellular matrices onto which cells can attach, grow, and form new tissue. The main focus of this paper is to understand cell behavior on micro-grooved and ridged substrates and to study the effects of geometrical constraints on cell motility and cell function. In this study, we found that BAE (Bovine Aortic Endothelial) cells naturally align with and are guided along 3D ridges and grooves machined into polymethylmethacrylate (PMMA) substrates. Average cell speed on micro-grooves and ridges ranged from 0.015 μm/s (for 12 μm wide and 10 μm deep ridges) to 0.025 μm/s (for 20 μm wide and 10 μm deep ridges). This compares with the cell motility rate on a flat PMMA surface where the average cell speed is around 0.012 μm/s. In this work we used scaffolds which were directly written with a focused proton beam, typically 1 MeV protons with a beam spot size of 1 Ã 1 μm2.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms - Volume 231, Issues 1â4, April 2005, Pages 413-418
Journal: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms - Volume 231, Issues 1â4, April 2005, Pages 413-418
نویسندگان
F. Zhang, F. Sun, J.A. van Kan, P.G. Shao, Z. Zheng, R.W. Ge, F. Watt,