کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9910115 | 1548649 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Changes in the mutagenic and estrogenic activities of bisphenol A upon treatment with nitrite
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Bisphenol A (4,4â²isopropylidenediphenol: BPA), an endocrine-disrupting chemical, is contained in food-packaging and can-coating agents as well as in dental sealants. Nitrite is present in vegetables, fish and tap water as an ingredient or contaminant, and also in human saliva. Here, we explored the possible generation of genotoxicity from the reactions of BPA and nitrite under acidic conditions, a situation simulating the stomach. We determined the changes in the mutagenic and estrogenic activities of BPA before and after nitrite treatment. Untreated BPA did not exhibit any mutagenicity. However, the mixture of BPA and sodium nitrite after incubation at pH 3.0 showed strong mutagenic activity toward Salmonella typhimurium strains TA 100 and TA 98 either with or without a metabolic activation system (S9 mix). The clastogenic properties of nitrite-treated and untreated BPA were analyzed by a micronucleus test with male ICR mice. A single gastric intubation of nitrite-treated BPA induced a significantly higher frequency of micronucleated reticulocytes (MNRETs) in mice. The results of analysis of electron spin resonance (ESR) suggest that the expression of the mutagenic activity of nitrite-treated BPA is related to the generation of radicals in the reaction mixture. By applying 1H and 13C NMR, AB-MS and APCI/LC/MS, we identified two compounds 3-nitrobisphenol A and 3,3â²-dinitro-bisphenol A. These compounds were synthesized by the reaction of BPA with nitric acid. 3,3â²-Dinitro-bisphenol induced a significantly greater frequency of MNRETs in male ICR mice. By applying a green fluorescent protein (GFP)-reporter expression system and an estrogen R(α) competitor screening kit, we found that nitrite-treated BPA and 3,3â²-dinitro-bisphenol A showed weak estrogenic activity compared to that of untreated BPA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 585, Issues 1â2, 1 August 2005, Pages 137-146
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 585, Issues 1â2, 1 August 2005, Pages 137-146
نویسندگان
Shuichi Masuda, Yumeko Terashima, Ayako Sano, Ryoko Kuruto, Yasumasa Sugiyama, Kayoko Shimoi, Kenichi Tanji, Hisashi Yoshioka, Yoshiyasu Terao, Naohide Kinae,