کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9913315 | 1550308 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Regulation of Streptococcus pneumoniae distribution by Toll-like receptor 2 in vivo
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کلمات کلیدی
CCDTLR2GBSGFPMFICFUStreptococcus - استرپتوکوکStreptococcus pneumoniae - استرپتوکوک پنومونیهGroup B streptococci - استرپتوکوک گروه BDistribution - توزیعCharge-Coupled Device - دستگاه متصل شارژCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیMeningitis - مننژیتmean fluorescence intensity - میانگین شدت فلورسانسcolony-forming units - واحدهای تشکیل دهنده کلنیgreen fluorescent protein - پروتئین فلورسنت سبزToll-like receptor 2 - گیرنده تله مانند 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The phagocyte pattern recognition receptor Toll-like receptor 2 (TLR2) and the multi-receptor adaptor MyD88 contribute to the reduction of bacterial load in infections with intra- and extra-cellular Gram-positive bacteria. Their mechanism of antibacterial action is mostly unresolved but evident in vivo by an increased pathogen burden in infected TLR2â/â and MyD88â/â compared to C57BL/6 wild type (wt) mice. We had previously observed higher bacterial numbers in brains of TLR2â/â than of wt mice with meningitis. Here we study bacteria-phagocyte interaction by comparing S. pneumoniae distribution and localization in wt and TLR2â/â brain by confocal microscopy using a green fluorescent protein-transformed encapsulated S. pneumoniae (C5017). Colony-forming units were similarly distributed in TLR2â/â and wt mice and exclusively localized in meninges and ventricles. Bacteria were more abundant in ventricles, in and around TLR2â/â than wt GLT1v+ plexus choroideus epithelial cells. S. pneumoniae were also found in and around Gr-1+ granulocytes, but never in F4/80+ macrophages, Iba1+ microglia, GFAP+ astrocytes, Meca-31+ endothelial cells or Neun+ neurons of either mouse strain. The results indicate that TLR2 does not change bacterial distribution, but may contribute to antibacterial defense by modulating S. pneumoniae adherence and uptake in plexus epithelia.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 210, Issues 2â4, 19 August 2005, Pages 229-236
Journal: Immunobiology - Volume 210, Issues 2â4, 19 August 2005, Pages 229-236
نویسندگان
Hakim Echchannaoui, Philipp Bachmann, Maryse Letiembre, Manuel Espinosa, Regine Landmann,