کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9914868 | 1551012 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Promoter cloning and characterisation of the transcriptional regulation of the human thyrostimulin A2 subunit
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Promoter cloning and characterisation of the transcriptional regulation of the human thyrostimulin A2 subunit Promoter cloning and characterisation of the transcriptional regulation of the human thyrostimulin A2 subunit](/preview/png/9914868.png)
چکیده انگلیسی
The novel heterodimeric glycoprotein hormone thyrostimulin consists of two unique subunits, A2 and B5. To understand its yet unknown transcriptional regulation, we characterised the 3.1-kb immediate 5â²-flanking region of the human A2 gene localised on chromosome 11q13. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides â506 to â347 relative to the ATG start codon. Interestingly, this minimal promoter appeared to be non-tissue-specific. Deletional, mutational and gel shift analyses revealed regulatory elements that are essential for the regulation of the A2 gene expression. Another noteworthy feature of this gene is the presence of silencer elements upstream and downstream of the promoter. To surmise, our results provide an initial step toward a detailed analysis of the underlying molecular mechanisms of the human thyrostimulin gene expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 245, Issues 1â2, 21 December 2005, Pages 169-180
Journal: Molecular and Cellular Endocrinology - Volume 245, Issues 1â2, 21 December 2005, Pages 169-180
نویسندگان
Ekaterina Breous, Achim Wenzel, Ulrich Loos,